Brain cell somatic gene recombination and its phylogenetic foundations
نویسندگان
چکیده
منابع مشابه
Detection of somatic DNA recombination in the transgenic mouse brain.
A DNA construct containing the bacterial beta-galactosidase gene (lacZ) was used to study somatic DNA recombination in the transgenic mouse brain. Recombination-positive areas of the adult brain were stained blue with X-gal, a substrate of beta-galactosidase. Blue-colored cells appeared soon after birth, and continued to emerge in postnatal tissue. Staining was prominent in sensory as opposed t...
متن کاملA complete immunoglobulin gene is created by somatic recombination.
Using a pCRI plasmid containing an enzymatically synthesized, full-length DNA transcript of immunoglobulin lambda chain mRNA as the hybridization probe in the Southern gel blotting experiments (Southern, 1975), we identified three DNA fragments of 8.6, 4.8 and 3.5 kb in Eco RI-digested total DNA from BALB/c mouse embryos. A fourth fragment of 7.4 kb was found in addition to these three fragment...
متن کاملGenetic Modulation of T Cell Receptor Gene Segment Usage during Somatic Recombination
Lymphocyte antigen receptors are not encoded by germline genes, but rather are produced by combinatorial joining between clusters of gene segments in somatic cells. Within a given cluster, gene segment usage during recombination is thought to be largely random, with biased representation in mature T lymphocytes resulting from protein-mediated selection of a subset of the total repertoire. Here ...
متن کاملSingle strand conformation polymorphisms (SSCPs) in the 5'- flanking region of Lactoferrin gene and its association with milk production traits and somatic cell score in Holstein cattle
Lactoferrin (Lf) is a bioactive protein present in all external secretions such as milk that plays important role in the innate host defense. Therefore, the Lf has a major role in bovine mammary gland defense during mastitis. In this study, 5’-flanking region of bovine Lf gene containing three single nucleotide polymorphisms (SNPs) at positions −602 (T/G), −600 (A/G) ...
متن کاملSingle Nucleotide Polymorphism in the Promoter Region of Bovine Interleukin 8 Gene and its Association with Milk Production Traits and Somatic Cell Score of Holstein Cattle in Iran
Background: Interleukie-8 is a proinflammatory cytokine and a potent chemotactic and activation factor for neutrophils. It has been reported to be expressed in high levels by various cell types after immune stimulation and mediates neutrophil recruitment in host. Objectives: The aim of this study was to analysis of the relationship between single nucleotide polymorphisms (SNPs) at position -18...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2020
ISSN: 0021-9258
DOI: 10.1074/jbc.rev120.009192